FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459852
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59840.17295537496979638No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG112500.020.056135
CGATCTT52750.019.6209815
CCGATCT282300.017.8914624
CTAGACG8350.017.3531553
GATCTGG38200.017.2501136
TTCCGAT296750.017.1367592
TCCGATC296550.017.1364353
GGACCGT17750.017.1043366
ATCTGCG9100.016.6814277
GTTATCG6000.016.67510811
CTTCCGA313100.016.5619091
TCGAACG2955.366019E-1016.37322417
GCGTAAC7850.016.26101752
ACCGTTA17700.016.178078
AGGACCG19450.016.1414935
CGATACG2151.2770888E-616.04638369
GATCTTT37400.015.9586596
CGCCGTT17350.015.90788625
TAGACGT12850.015.8403414
GACCGTT18050.015.6732327
Produced by FastQC (version 0.11.2)