FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946122
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG1258.743668E-519.32038112
CGATCTT30950.019.1727475
ATCTGCG5250.018.399897
TGTCGCG950.00783662118.15778710
TCGGTCA950.00783662118.15778768
CGATCTG64900.018.0206935
TCTGCGC3400.017.2498978
TTATACT8400.016.4284734
GTGCCGA2109.984051E-716.42847366
GCCGACC1907.024404E-616.34200964
CCGATCT163950.016.2871864
CCGAACA1503.526858E-416.09990552
CTGTCGC4100.015.9877099
TTCCGAT170850.015.7101782
GTATTAG7050.015.6594811
TCCGATC173700.015.4921363
GATCTGG22050.015.3332426
ATCTCGT4750.015.2525427
AGCGGCA1605.7608704E-415.09365948
CTTCCGA181550.015.050311
Produced by FastQC (version 0.11.2)