FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999345
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37120.1856608039132816No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAC800.002900283321.5623593
CGATCTT33750.020.6487545
ACCGGTA850.00412138720.29398515
GCCGATA850.00412138720.29398547
CGACCGT900.005736085619.16654248
CGATCTG70200.018.1836415
AGGTCGC1552.2475126E-517.80633568
CCGATCT176550.017.5088064
GATCTGG23650.016.9216656
TTCCGAT183650.016.8323292
TCCGATC186700.016.6493283
GATCTTT24050.016.6402246
TTACGTG10350.016.33322768
TACGTGT10200.016.2351969
TAGACGT7250.016.1792054
ACGGCCG5400.015.97251820
CGACTCC45200.015.9523369
CTTACGT13650.015.92297369
CTAGACG5000.015.8698973
CTTCCGA197700.015.8288541
Produced by FastQC (version 0.11.2)