FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006916
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13250.13158992408502795No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA450.00462100630.66858720
GCACCGT756.160138E-527.6017286
TACGATG500.00773585527.59898836
TTCGCGG500.00773585527.59898869
CGTCGTA1555.2750693E-1126.70869633
GACGGTC809.556605E-525.8766218
CGACGGT851.442513E-424.3544677
GTCGTAA1701.6734703E-1024.35204734
TAGCGCG750.001993277622.99915532
TTACGAC1704.436515E-922.3238184
GTTAACG1103.2673182E-521.95374166
ACGACGG953.063959E-421.7908386
CGGTCCA953.0649878E-421.78975510
GCGTCGT953.066017E-421.78867132
TACGACG1756.195478E-921.6870735
CCGCAAA2101.0186341E-1021.3563664
CGATGTA1206.38788E-520.1242638
ACGAATT2402.7284841E-1120.1242647
GACCGTC1056.021753E-419.7145430
CTCGCGT1056.021753E-419.7145429
Produced by FastQC (version 0.11.2)