FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92221
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27723.00582296873814No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA150.002348664269.0000129
CCTACTC150.002348664269.000013
CATCTTA150.002348664269.0000167
TCTCGAG150.002348664269.0000116
CCCTACT200.007337701551.752
CTAACAC200.007337701551.753
AGTTCGG200.007337701551.7552
GTATCCT200.007337701551.7514
TCCCGGG200.007337701551.7564
TACACCG200.007337701551.755
GGTAGAC200.007337701551.756
GACTACC200.007337701551.757
GTATAAT352.256505E-549.2857171
CCGCTAC306.2811474E-446.00000438
TATTCCA306.2811474E-446.0000045
GTCGTGC306.2811474E-446.00000440
TTCGTCT350.001341385839.42857425
GCACCTT350.001341385839.42857420
TTTATCC350.001341385839.42857443
CTACTCC400.002584020634.54
Produced by FastQC (version 0.11.2)