FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133190
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71035.332982956678429No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGAG200.007348465351.74352344
CCGTGTG200.007348465351.74352317
GTTACTA200.007348465351.74352361
CTCTCAC200.007348465351.7435234
ATCGGAG306.2951515E-445.99424419
CTTTATC459.975405E-538.3285333
GTGTAGA709.631694E-734.5086469
TCCAAGC553.2298113E-431.383282
CTCCAAG450.00459407330.6858751
CCTCCAC450.004611001330.66282839
GTCAGAG500.00771549727.59654437
AGTGTAG1404.5656634E-1027.11393269
GAGTGTA2250.024.53947469
AGAGTGT2550.021.65248168
TCGAGCA900.00568775319.178672
GAGAGTG3400.018.2692869
CAGGTCG950.00780470818.15562250
AGAGAGT4301.8189894E-1214.44547768
ACGGAGA9400.013.95030269
GACGGAG11000.011.92116668
Produced by FastQC (version 0.11.2)