FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24349
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18017.396607663559078No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAG150.00232783169.041
TGATCAT150.00232783169.04
CTCACGG150.00232783169.035
CAGTCGA200.007273116651.7557
AGTCGAT200.007273116651.7558
GTTGGGG200.007273116651.751
GAATTAG1206.064491E-520.12567
ATTAGAC1107.924428E-418.81818269
AATTAGA1301.1218202E-418.57692368
CGAATTA1300.002427744415.92307766
Produced by FastQC (version 0.11.2)