FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451801
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31130.1269678901346398No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGA850.00412077420.2947323
CGGTCAT850.004121260720.29431519
CGGATTA3400.019.27920762
AGGACGG3800.018.15770368
GTTATCG2156.601658E-817.65097454
CTAGACG15200.017.2498173
TAGACGT20600.016.914874
CGAAATA3700.016.78360653
GTATTAA8250.016.7284581
CGTCGTA1300.002522984515.92290733
GCGACAC2654.177855E-815.62247545
CGAAGGC58000.015.34638869
GTCGTGA1605.758264E-415.0948221
GAGGACG5300.014.97153867
CGATCTT55600.014.9542825
GGACGCG1851.0221562E-414.9187669
TACGACG1400.004132345314.7858595
CGACCAA2605.727943E-714.59599866
CGATCTG150200.014.5627255
TAGGGAG40300.014.5531954
Produced by FastQC (version 0.11.2)