FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363907
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20850.15286966046805245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAA500.0077364627.59923711
CGATCTT20650.022.720925
GCCGATG1004.3361852E-420.6994298
CCGATGC850.00412123120.2935569
CGTTTAA850.00412123120.29355614
CGATCTG41650.019.3824185
ATACCGA900.005735868619.1661386
CCGATCT111700.018.0062184
TTCCGAT115900.017.5329442
GATCTTT18200.017.439086
TCCGATC118300.017.1182943
CTTCCGA124850.016.6641671
GATCTGG15650.016.5330896
ATCTTCG2301.4237412E-716.4995447
GTCGCCC1907.022758E-616.34165411
TTATACT5550.016.1617164
ACTGATC8400.016.0174148
CCGATCC3251.2369128E-1015.92322133
ATCTCCG3950.015.7210847
TGTCGCC2051.4435345E-515.14592310
Produced by FastQC (version 0.11.2)