FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951518
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21110.10817220235734437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG36650.021.8393275
CGATCTT22600.021.524645
CCGATCT94650.020.3394324
TTCCGAT98950.019.5606562
TCCGATC99850.019.3838483
CTTCCGA106100.018.6661641
TCGCAAT950.0078368818.15768459
ATCTGCG4600.018.0002547
GATCTGG19250.017.7431076
TCTGCGC2251.1085285E-716.8669058
GCCGACC1250.001941414816.5598169
TTACCCG1250.001941414816.5598148
GTATTAA8100.016.1866571
GATCTTT20550.015.2775886
TAATACT9100.015.165054
TTTCGGA1851.0211021E-414.9202761
CGTACAC1400.00413182514.7859233
GTCCGGG1400.00413252914.78554412
GTATAGA5650.014.65621
TTTCGAG1657.275373E-414.63619611
Produced by FastQC (version 0.11.2)