FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2116343
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29720.14043092258674517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT26750.024.2470285
CGATCTG49400.024.2341065
CCGATCT121150.023.3509524
TTCCGAT125900.022.744522
TCCGATC126950.022.5286943
CTTCCGA138850.020.8970411
TAGACCG1851.1776137E-820.5137675
GATCTGG20100.020.253986
TTACGTG11250.019.31978268
TACGTGT12500.017.93979869
ATCTGCG5050.017.7625967
CGTGTAG5050.017.76217769
CGATCTA19700.016.812395
CGCACGA3106.002665E-1116.6933642
CGATCTC40550.016.6759155
GATCTTT24950.016.4551126
CTTACGT14200.016.0350367
TACCGCC3058.60382E-1015.8362617
ACGTAGT3502.1827873E-1115.77162423
ATACCGC3502.1827873E-1115.7716246
Produced by FastQC (version 0.11.2)