FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34436
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24026.975258450458822No Hit
ACACACACGTCTAGCGATATACATGTGTACGTCTGAGTGCTCGCTGACGTGACTGTCTCTTATACACATCTGACG910.26425833430131257No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTGTA150.00233612469.023
CGTCTAG150.00233612469.08
CTGACGT150.00233612469.044
TCTAGCG150.00233612469.010
TACATGT150.00233612469.020
ATCTTGC150.00233612469.032
GTGTACG150.00233612469.025
AGCTCTT150.00233612469.038
CACGTCT150.00233612469.06
TATACAT150.00233612469.018
TACGTCT150.00233612469.028
CCTCTTA150.00233612469.01
CTAGCGA150.00233612469.011
CTGAGTG252.532231E-455.233
GAGTGCT200.007298824451.7535
CACGGCC200.007298824451.757
TGGGTGC200.007298824451.7548
CGTGACT200.007298824451.7548
ACACACG200.007298824451.753
TGAGTGC200.007298824451.7534
Produced by FastQC (version 0.11.2)