FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55580
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21273.826916156890968No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAC150.002343735769.039
TGCTTGT200.007322420451.75000410
GGTAAAC200.007322420451.75000423
ACCATTG200.007322420451.75000444
TTTAACG200.007322420451.7500045
CATGAGA200.007322420451.75000412
TAAACGT200.007322420451.75000425
GGCCCAA200.007322420451.75000426
GTAAACG200.007322420451.75000424
AGGCCCA400.002575210534.525
ACATCCT450.004585311730.66666651
GAAGTGG500.00767275527.617
ACGGAGA2704.9452865E-815.33333369
GACGGAG2951.4634134E-714.03389868
GGAGAGA1950.002509996312.38461469
CGACGGA3459.812629E-712.067
CGGAGAG2050.003650790311.78048868
ACGACGG3853.6738438E-610.75324766
GACGACG4906.397784E-58.4489865
CGACGAC5452.4427452E-58.22935868
Produced by FastQC (version 0.11.2)