FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112923
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30970.14657420076358676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT43300.020.9552885
CGATCTG88500.019.5697925
ACGGTGT12350.018.15774569
GATCTGG31050.017.7780536
CCGATCT227050.017.7631024
ATCTGCG7400.017.716077
TTCCGAT234200.017.3537962
GATCTTT30650.017.2221266
TCCGATC238350.017.0512393
AAGTCGC1855.4517004E-616.78364658
CCTATCG1452.7239433E-416.65503529
TCTGCGC5200.016.5864038
CTTCCGA250100.016.4448341
ACGATTG1704.9633927E-516.23554421
TTTCGGA4000.015.52671051
CGAATTA50350.015.41695469
CGTAAAC2705.2532414E-815.333570527
AACGTCG1350.003244933415.33284454
GACGGTG13900.015.14016468
CGCTTAG1657.274242E-414.63658934
Produced by FastQC (version 0.11.2)