FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31491
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29979.517004858531008No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT150.00233425169.025
CCGAAGT150.00233425169.044
GCACTTC150.00233425169.06
CACTTCG150.00233425169.07
AACAATG200.007293018551.757
CACGCGT200.007293018551.7552
GTAGGAG200.007293018551.754
CCTCAGC200.007293018551.7556
AGACAAT200.007293018551.756
GAGCCAA200.007293018551.756
TCTCCCA200.007293018551.7510
TAGGAGG200.007293018551.755
CGCAAGT200.007293018551.7519
CCAGCAC306.2178244E-446.03
CGAATTA1350.003150367915.33333369
ACGAATT1550.00788277913.35483968
GACGAAT1850.001661405113.05405469
GCCGACG2752.1361688E-411.29090966
CGACGAA2200.006081986710.97727268
ACGCTGC4100.0081864357.57317169
Produced by FastQC (version 0.11.2)