FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2972210
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36730.12357807826499474No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCG703.853399E-529.57103248
CGATCTG96300.019.7754365
CGATCTT46200.018.6685815
GATCTGG32950.017.7994586
CCGTGCG1602.9532337E-517.249779
ATCTGCG7800.017.2497677
TAGACGT12450.016.6262824
CCGATCT248800.016.3484544
TTCCGAT259450.015.9305612
CTAGACG9550.015.8950743
TCCGATC262650.015.7490743
CTGCGCG2201.622846E-615.6816089
CGAAGGC63450.015.55114269
CGTGCTA1350.003244949715.33312710
GTATTAA11300.015.2668231
CTTCCGA274600.015.1783131
GTATAGG6900.015.0013121
TAATACT10600.014.97149854
TCTGCGC5350.014.8315768
GTATATA23050.014.0706461
Produced by FastQC (version 0.11.2)