FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042675
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24050.1177377703256759No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG1057.688577E-726.285675
TTTCGGA1906.7484507E-1021.7915691
TTCGGAA2252.564775E-1019.9337862
CGATCTG55400.019.8032155
CGATCTT29600.019.3479395
ATAGGCG1258.7453795E-519.3199675
GGGTACG950.00783656218.1578627
GTATAAG6550.017.910121
TATACTG7800.017.6922765
TCTGTCG4500.017.6333038
CCGAAAA10300.017.41744417
CCGATCT150650.017.0152384
TCCGAAA10850.016.85250516
GGGGTGC11550.016.4285436
CTGTCGA1704.963994E-516.2352669
TTCCGAT159750.016.1111622
TAGACGT5800.016.0603164
TCCGATC160700.016.0369973
CGAAAAA11650.015.99138818
GTATTAT8750.015.7729441
Produced by FastQC (version 0.11.2)