FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931389
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24270.12566085858415885No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGAT850.004121369720.29395931
TACGCTT1056.025343E-419.7141325
CGATCTG72500.019.7005355
CGCCGAT1258.745474E-519.31984966
CTCCGCG900.005736060419.1665159
GATCTGG24150.018.714146
TGTCGCC2851.6370905E-1118.15775110
TCTGCGC3205.456968E-1217.2498658
CGATCTT29850.016.8742395
ACTGATC12300.016.8291368
TAGACGT8650.016.7513144
CCGATCT180100.016.2250254
GTATTAC4800.015.8136041
ATCTGCG8300.015.7950567
GTTACGA1756.360621E-515.77130449
CTAGACG6650.015.5637873
TTCCGAT190050.015.4485822
TCCGATC191400.015.3031723
CGATATG37400.015.220468569
TTCGGAA2051.44353E-515.1466142
Produced by FastQC (version 0.11.2)