FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480525
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27950.11267775974844035No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG66450.020.559745
CGATCTT37700.019.4919685
GATCTGG24300.018.456726
CCGATCT185750.017.3474454
TAGACGT8100.017.0369724
TTCCGAT194350.016.598912
TCCGATC195500.016.5175843
GATCTTT28250.016.4866626
TTACGTG9900.016.37872568
CGTGTAG4900.016.19381569
GGGGTGC15600.015.7018646
CTTCCGA207700.015.6997071
GTATTAG9100.015.5467171
TACGTGT10700.015.47657669
CGACTCC44350.015.40242369
CTGTGCG2705.255788E-815.3332759
CCGAAAA13950.015.08657317
GTGTATA16950.015.0646231
GGCGCGA2302.5770587E-614.9996433
ATCTGCG5300.014.9716427
Produced by FastQC (version 0.11.2)