FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327379
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25460.10939344215102052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG2300.024.0004564
ACGACGG2750.022.5822475
CGACGGT2650.020.8305846
CGATCTG77150.019.4527345
CGCGTAT900.005736228519.16661857
ATACGAC2901.8189894E-1219.0348433
GCGTTAT1108.239134E-418.8185415
TGTACCG1903.500627E-718.158245
CGATCTT35450.017.3233185
GATCTGG26400.016.8582746
GTACGCC3106.002665E-1116.69350669
CCGATCT196800.016.3562564
AACGCGG6200.016.13740221
GAACGCG6150.015.70761620
TTCCGAT208700.015.6557382
TACCGCG1554.5259474E-415.580947
GCGAACT6650.015.5645427
TCCGATC209200.015.55168153
GCGAACG6700.015.44805418
CGAGATT6050.015.39665569
Produced by FastQC (version 0.11.2)