FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225787
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49012.1706298413991947No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGGT150.002353089269.000015
CGTTAGC200.007351416651.75000436
GTCGTAC200.007351416651.75000429
TCGTACC400.002591936234.530
GGATAAG652.3133241E-531.8461531
CTCTAGT553.2488062E-431.3636381
AGACTCA450.00461490830.66666646
GAAGTAC450.00461490830.66666636
AAGGGTT450.00461490830.66666659
CTAGTGG500.00772197327.641
ACTACAT500.00772197327.63
CCCGACT500.00772197327.649
AAGAGTA658.635466E-426.53846226
GATAAGG700.00133053124.6428592
GTATAAT700.00133053124.6428591
GTTGTCG750.001988093823.09
GCACCGA1004.3199566E-420.76
ATAATAC850.00411009820.2941173
GCTATGC900.005720454319.16666630
GACAAAG900.005720454319.16666642
Produced by FastQC (version 0.11.2)