FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31471
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26388.382320231324076No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACA150.00233425169.06
GCTGCAG150.00233425169.045
TGTACGC150.00233425169.08
GTCGGTA150.00233425169.066
CGACATT200.007293018551.758
AGAAAAG200.007293018551.7563
GAATTAG1653.6538513E-516.72727269
CGAATTA1756.0480255E-515.77142968
ACGAATT2001.8868709E-413.867
GACGAAT2153.481346E-412.83720966
CGACGAA2408.788811E-411.565
GCCGACG2954.0937797E-410.52542363
CCGACGA2800.00317724869.85714364
TGCCGAC3150.0083870798.76190562
Produced by FastQC (version 0.11.2)