FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945286
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46040.1563175868149986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG8050.019.7145823
TAGACGT12100.018.8184644
GATCTGG33300.018.7525336
CGATCTG98550.018.589325
CGATCTT47850.017.8811455
AATCCGC1200.001477707117.24967449
TACCGTA2109.989672E-716.4282649
CCGATCT257800.016.2733234
ACCGTAA2351.820481E-716.1486350
TTCCGAT271350.015.5626872
TCCGATC272850.015.4768683
TGGTCCG2252.0507523E-615.33304310
GTACCGA1605.7611987E-415.0939776
CTTCCGA287100.015.0456691
ATCTGCG7900.014.8483257
TAATACT11450.014.4630994
CGAAGGC57700.014.17044269
GTATTAA11800.014.0345861
TAAGGCG2751.0787535E-613.8002075
GTATAAG10050.013.7320171
Produced by FastQC (version 0.11.2)