FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404957
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34120.1418736384891705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGC4100.021.8780238
CGATCTG88300.020.707795
GATCTGG28550.019.8178396
GTATTAG7150.018.8189411
GGACCGT1150.001110712117.9999796
CGCGGGA1954.598278E-717.69228613
CGATCTT43650.017.6254085
GGCGTCC1200.001477424817.2499798
CCGATCT237850.017.2028394
TTCCGAT249750.016.5081732
TCCGATC252500.016.3413683
ATCTGCG8500.016.2352757
ATCTCGC8850.015.9830317
CTTCCGA266050.015.7820681
TGTGCCG2855.989932E-915.73682310
CTGCGCG2201.6223257E-615.68189
TAGACGT8600.015.645334
CGAAGGC57100.015.58842269
TTATACT10200.015.55880454
CGATCAT4500.015.33331534
Produced by FastQC (version 0.11.2)