FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395536
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25160.10502868669057781No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG1251.2657802E-724.83987269
CGATCTG62750.018.5831725
CGATCTT30400.017.590375
TAGACGT8700.017.4481874
CCGATCT165600.016.7290824
GAGCGTC1250.001941505916.5599167
CGTCCGC1250.001941505916.55991659
CCGATCG3058.60382E-1015.83598533
TCCGATC176150.015.7858973
TTCCGAT175650.015.7525962
GTATATA15900.015.4062231
GTATTAA8750.015.3777061
CTTCCGA185100.015.2469121
ATAATAC10700.014.8316993
CGATCGT1400.004132719214.78563834
CCTATAC6350.014.6692163
GTCTTAG7300.014.6512211
TATACTG8550.014.5262415
GATCTGG22750.014.4065196
GTATAAG8700.014.2763831
Produced by FastQC (version 0.11.2)