FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4915205
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71700.14587387504692073No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT147200.035.649242
CGATCTG71850.035.4364055
CCGATCT146050.035.2914164
TCCGATC149800.034.937663
CTTCCGA157950.033.1799621
TCTTCCG83650.024.498565
GATCTGG45800.024.1801836
CGATCTA21150.022.0213265
GTGCTCT97100.021.7099951
CGATCTC56650.021.558745
GCTCTTC96850.021.1951943
CGATCTT47250.020.9556035
TGCTCTT105750.019.6727832
ATCTGCG10200.018.9412177
CGCGTCG7500.018.8598531
GCGTCGT7500.018.8598532
TCGCGTC7800.018.57696330
CTCTTCC116900.018.1501674
ACCGGTA5350.017.4114315
CGACGGT8750.017.348617
Produced by FastQC (version 0.11.2)