FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278130
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32320.14187074486530618No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTT43300.022.3093855
GGTCGCG800.002900361821.56241669
GCGCATA850.004120972520.29448521
CGATCTG67650.020.2460425
GATCTTT35350.019.323836
GTATTAG9300.018.5483171
CCGATCT189900.017.7495384
TTCCGAT195300.017.382042
TCCGATC199050.017.0722793
TAATCCG1855.451846E-616.7837248
TAGACGT8750.016.1656534
CTTCCGA213950.016.1090761
GTATAGA8150.016.0858291
CTGTGCG2201.6222784E-615.6817599
TCTGCGC3301.5643309E-1015.6817588
CGTGTAG5300.015.62258269
GTCCTAT6050.015.3966361
TGTGCGG1808.091202E-515.33327610
GATCTGG26300.015.2166726
ATCTCGT5250.015.1142277
Produced by FastQC (version 0.11.2)