FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13200765
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG387150.018.8205155
CGATCTT170000.018.7110795
GATCTGG141350.017.1339766
CCGATCT984400.016.548984
TTCCGAT1018350.016.088982
TCCGATC1043900.015.7379193
CTTCCGA1059200.015.6352491
CGAAGAG263150.015.35219169
TAGACGT44750.014.339594
GTATATA95700.013.8800271
GATACCG12250.013.7999296
GTATTAT63700.013.7032191
GTGTATA88400.013.5821491
GTATAGG47350.013.4801041
ATCTGCG39550.013.2590967
ACCGTTA11200.013.2454678
GTATTAA54600.013.2067251
GGACCGT12300.013.1828586
CGGACGG11800.013.15676230
CGCCGTT9450.013.14308825
Produced by FastQC (version 0.11.2)