FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43701
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34757.951763117548798No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACCC150.002340376469.02
CTCCCCC150.002340376469.052
CCGCTCC150.002340376469.049
TACGCGC150.002340376469.036
CTACGCG150.002340376469.035
GCCCCGA200.007312008651.7556
TGCCCCG200.007312008651.7555
TATTGGT200.007312008651.7547
GGAAGTT200.007312008651.7555
GGAAGTA200.007312008651.7564
TCAGCTA200.007312008651.7546
CTTGCTA200.007312008651.7551
AACTGTA200.007312008651.7532
AACGAGA200.007312008651.751
GCGGCTA200.007312008651.7531
TGGATCG200.007312008651.755
GCTACGC200.007312008651.7534
AGTACAG200.007312008651.7567
ACGCGCC200.007312008651.7537
CAATAAC306.2447105E-446.03
Produced by FastQC (version 0.11.2)