FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171692
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68724.002516133541458No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGCT150.002352126469.031
TACTACT150.002352126469.037
TGCGTAG208.4936044E-569.02
CTAGAAG200.00734843151.753
CCTAGTA200.00734843151.752
GTCCTAG200.00734843151.7520
CACTGAT200.00734843151.7531
TATTAGC200.00734843151.7567
ATAGTCT200.00734843151.7563
CTCTAGA306.296388E-446.01
TACCTCC404.984184E-543.12534
CAAAACG350.001344620139.42857444
TAGGGAT350.001344620139.4285745
GTCTGGG400.002590212334.525
CTATCCT400.002590212334.532
GTGTAGC400.002590212334.51
TCCAAGG501.8548465E-434.553
TATACTC400.002590212334.55
ATCTATC553.2455867E-431.36363430
ATGAGTG553.2455867E-431.3636349
Produced by FastQC (version 0.11.2)