FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40450
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG472011.668726823238567No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAT150.002339111969.0000114
GGCCCGT150.002339111969.0000141
GATGGCG150.002339111969.0000167
CTACGAG150.002339111969.000014
GGGTTTC150.002339111969.0000128
TTGTTAC150.002339111969.000018
AGGATTA150.002339111969.0000165
GGATTAG150.002339111969.0000166
GGTTCCT150.002339111969.0000128
CAGAGCA150.002339111969.000014
AAAGATC150.002339111969.0000137
AAGATCA308.968571E-657.50000438
ATGTTGT252.5375988E-455.210
AGTCCCC200.00730808851.7500041
TTAATTG200.00730808851.7500044
CTCAAAG200.00730808851.75000434
GGCGTTC200.00730808851.75000411
AAAAGCC200.00730808851.75000411
TCGATGA200.00730808851.75000412
ATCAAAA200.00730808851.75000441
Produced by FastQC (version 0.11.2)