FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2689325
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42720.15885026911957462No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG69100.022.766585
CCGATCT191250.019.2474864
TACGCGG900.005736372419.16666610
TTCCGAT201300.018.423652
TCCGATC201950.018.347273
CGATCTT44450.018.0840285
GATCTGG27850.017.9619646
CTTCCGA215450.017.566911
GGACCGA1200.001477658517.249686
TGTACCG1804.2011525E-617.249685
TACGAGT1300.00252320315.9227814
GTATTAG8050.015.8577331
CGCGGGA1756.360668E-515.77172213
TAGACGT7250.015.7031564
CGGATTA2252.050585E-615.33304857
ATCTCGC7450.014.8185187
TATATAC21200.014.6459543
GTACTAG3554.6929927E-1014.5780071
TGTCGCC3554.6929927E-1014.57746510
CTTCGCG1450.005217289614.2758639
Produced by FastQC (version 0.11.2)