FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419107
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41140.17006275456191064No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG74750.019.8919095
CGATCTT35250.018.5953715
TGTCGCC4900.016.89796810
CCGATCT196000.016.7216034
CACGCGT1250.001941707716.55966851
TATACTG11000.016.3087645
TTCCGAT205850.016.1390082
TCCGATC207350.015.9393963
CTAGACG7250.015.7031343
CGATACT48750.015.63968769
GTATAAT9500.015.6154761
CTTCCGA218200.015.4946481
GATCTGG29450.015.3460436
CCGATCC6600.015.15910133
CTGTCGC6400.015.0937599
TAGACGT10350.014.9996994
GTCGCCC4850.014.93815311
CGATACA2554.598587E-714.88205469
GTGCGTT2101.8132729E-514.78602913
GTATTAT11550.014.636071
Produced by FastQC (version 0.11.2)