FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5021221
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67630.13468835568081947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG129900.020.3704175
CGATCTT64000.018.543525
CCGATCT323750.017.9131374
TTCCGAT344300.016.9346542
TCCGATC345600.016.8405043
CTTCCGA360550.016.2771531
GATCTGG51450.015.4225496
TAGACGT18650.014.9837284
ATAATAC21600.014.8539823
ATCTGCG13150.014.4294797
GTATTAT19950.014.3540631
GTATAGA18850.013.9104731
CTAGACG13150.013.9047713
GATCTTT48050.013.8572676
CGACCCG3506.01176E-913.79996637
TCGTTAG20450.013.66486611
GTATATA37700.013.5444081
CGATATG104450.013.47627269
ACGATAT134500.013.36390869
GGATCGT17850.013.3359698
Produced by FastQC (version 0.11.2)