FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17748442
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239110.1347216843033321No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATG195460.11012797630349752No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCA184430.10391334630949578No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA61350.023.11266315
CCGGTAA66900.021.19530516
CGGTCCA75000.019.13578410
ACGCTTA76000.018.97548324
GATCTGG190150.018.9237446
TACGCTT76250.018.8680223
CGATCTG570500.018.6015785
CCGATCT1280150.018.4472524
AATACGC77900.018.3355121
ACGGTCC78450.018.1185479
TTCCGAT1324200.017.9096072
TAATACG80050.017.88584920
CGCTTAC80700.017.87034225
TCCGATC1343000.017.661023
GCGTCGT78900.017.57779932
CTTCCGA1383550.017.3341331
TCCACCG83600.017.16736812
TCGCGTC80950.017.00498830
CGCGTCG81450.016.94276831
GACGGTC86000.016.8087228
Produced by FastQC (version 0.11.2)