FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337981
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42840.18323502201258265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG6200.021.70160769
CGATCTG81300.020.708485
CGATCTT37200.019.6612855
GATCTGG28850.018.774696
CCGATCT205000.018.512194
TCTGCGC3600.018.208338
TACGTGT13700.017.87955569
TTACGTG13750.017.8145468
TTCCGAT215750.017.7337152
TCCGATC218900.017.4942843
TTGTCGG5200.017.24999422
CTTCCGA226950.017.0109351
AGTACCG3309.094947E-1216.7272685
GCACGAA5400.016.61110743
AGTTCCG5400.016.61110732
CGGGAAC2552.6022462E-816.2352915
TCCGACC5600.016.0178535
TCGGGTA5200.015.92307225
ATCTCGC7850.015.8216517
TGTCGGG5950.015.655457523
Produced by FastQC (version 0.11.2)