FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897765
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21040.11086725701021992No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA756.1646824E-527.60060121
GCGTAAA1052.281408E-523.00050222
GCGCGTA800.002899753621.5629720
TAGACCG1004.3363706E-420.6999075
CGGATAG1301.18158256E-418.57683869
CGATCTT26500.017.5753925
TTATACG1704.9638144E-516.235224
ATACCGG1300.002522615.9230046
CTAGACG6300.015.880883
CCGTAAG1554.5253037E-415.58098413
CGATCTG62700.015.2965815
TAGACGT8050.014.9999314
CCGATCT156350.014.8502674
GACCGTG1400.00413228314.7856467
CTCGCAC1400.00413228314.7856469
GACGTTA1657.274861E-414.63629739
GATCTGG20300.014.6156966
TTCCGAT164950.014.34791852
TACGACG1450.005216701414.2757975
GTATATA13550.014.2586141
Produced by FastQC (version 0.11.2)