FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78215
Sequences flagged as poor quality0
Sequence length75
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2331929.813974301604553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACTT150.002347326869.012
CTCAAAA200.007333553451.7546
GGCCGTT200.007333553451.7518
TTGTAGA200.007333553451.754
TGAGTCT200.007333553451.7564
TGTTGAC200.007333553451.7550
TTCCCCG200.007333553451.7510
GTTGTAG200.007333553451.753
GTGTCCG200.007333553451.7542
ACATCGT200.007333553451.7533
GATTCTG200.007333553451.7529
AATGTTA200.007333553451.757
CGGGGAG306.275262E-446.05
CCGGGGA350.001340135939.4285744
AATGAGA350.001340135939.42857461
TGGTCGC756.091503E-527.59999869
GGGAGAT809.450603E-525.8757
GGGGAGA700.001322769724.6428596
GGAGATC851.4265825E-424.3529438
GGTGGTC851.4265825E-424.35294367
Produced by FastQC (version 0.11.2)