FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94137
Sequences flagged as poor quality0
Sequence length75
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76378.1126443375081No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAA150.002348815869.066
ATCAATG150.002348815869.08
CGCTAGC150.002348815869.065
GCGCTAG150.002348815869.064
GACCTAT150.002348815869.06
TAGAGCA200.00733816751.7500044
TTTACGG200.00733816751.75000430
TAGGTCG200.00733816751.7500045
ATAGCTA200.00733816751.75000463
GAATCGT200.00733816751.75000447
TTACTAT200.00733816751.75000443
TTATAGC200.00733816751.75000461
CGCAGTA200.00733816751.75000468
TATAGGG200.00733816751.7500045
TATAGCT200.00733816751.75000462
TACAGTT200.00733816751.7500045
CACCGTA200.00733816751.7500047
TAGCTAT200.00733816751.75000464
AATAAGG200.00733816751.7500043
ATACACG200.00733816751.7500046
Produced by FastQC (version 0.11.2)