FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65761
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG762811.599580298353128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCT150.002345658469.056
TTGTGTC150.002345658469.038
TTACTTA150.002345658469.020
TGGTTTA150.002345658469.010
AGACAAG150.002345658469.016
GCCTATG150.002345658469.068
GGTTCGA150.002345658469.035
TAATTTA252.5493707E-455.218
CTTCGGT200.007328382751.7518
GGCAGAA200.007328382751.7531
CAACGCT200.007328382751.7515
GGGTCAT200.007328382751.7538
TCACAAG200.007328382751.7528
ACCGATC200.007328382751.758
GAGACAA200.007328382751.7515
CGGTGCT200.007328382751.7530
GAACACG200.007328382751.755
GGGGCAA306.267924E-446.011
CTTGTGT350.001338578639.42857437
GGTCATA350.001338578639.42857439
Produced by FastQC (version 0.11.2)