FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411198
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38480.159588718968745No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG80600.020.6742825
CGATCTT39300.019.8396595
TGCGCTA2102.3537723E-919.7142510
ACGTACG900.00573626419.16663258
TAGGTCG1108.240406E-418.8181485
CCGATCT212200.018.4855944
TTCCGAT222650.017.7426742
TACGGCG3400.017.2499712
CTTCCGA234900.017.0677971
TCCGATC233400.016.9395583
GATCTGG27500.016.9363336
TAGGACG7800.016.80766312
GCGCTAT2301.4246507E-716.4999711
CGCGGTT5650.016.48669420
TGGTACG2753.7325663E-916.30906110
GTCGTAA1300.002521423615.9243681
TCTGTCG5450.015.825668
CGCCGTA7650.015.78428649
ATCTGCG8100.015.7592317
TCGCGGT6050.015.39666719
Produced by FastQC (version 0.11.2)