FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10421423
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145060.1391940428864657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG201950.023.9340085
CGATCTT186700.023.7823835
CCGATCT603100.022.195434
TTCCGAT623800.021.5640872
TCCGATC633350.021.2061483
GATCTTT179700.019.736326
CGATACA6500.019.107669
CTTCCGA738100.018.5760151
CGATACG4400.018.03400469
ATCTGCG23600.017.6886447
GATCTGG95450.017.2410456
TTCGGAA13750.017.0619812
TCTGCGC15400.017.0260548
TTTCGGA14600.016.77821
ATCTTTT211700.016.459697
GGGGTGC115250.016.1356
GATCTCG68750.015.3557086
TCTGGCG14850.015.33340558
CCGATCC25700.015.30335333
TGTCGCC14950.015.23062110
Produced by FastQC (version 0.11.2)