FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2055552
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34100.16589217884052557No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT1103.268304E-521.9547398
CGATCTG38800.021.7849865
CGATCTT28000.021.1930455
CCGTATA1056.024848E-419.714469
CCGATCT108650.019.5275234
TTCCGAT112350.019.0998362
TCCGATC116750.018.3795683
CTTCCGA124400.017.3337731
GATCTTT26400.016.8581036
GTATTAT7900.016.1587641
TCGGTTA1300.002522061315.92360324
ACACCGT1756.360031E-515.7715676
GATCTGG18300.015.4591536
CTGACCG1808.089719E-515.3334689
CTAGACG3151.3606041E-915.3327243
CGCGGGA1605.759356E-415.09425113
GTATAGG4850.014.938641
TTATACT8100.014.9068134
GGGGTGC14600.014.8871176
GTATAAT9800.014.7862051
Produced by FastQC (version 0.11.2)