FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899368
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41300.2174407487122032No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGTA1001.45046215E-831.05111520
CGCGTAA1001.45046215E-831.05111521
CGCTCGT450.00462382230.66615132
CGCTAAC658.660036E-426.53801560
CGATCTT49250.026.3393365
ATACCGT700.001334092524.6430936
CGATCTG44250.024.247695
GTACCGT1001.5642441E-524.1502326
GATCTTT47450.023.2668176
CCGATCT139300.023.2561444
TTCCGAT144850.022.4609382
TCCGATC147000.022.1553133
GATCTGG16950.021.778976
TGTACGG800.002899518221.5632722
ATCTTTT51900.021.0059817
TCGCACG850.004121558320.29377610
GGCGCGT1551.0048298E-620.03297819
ATCTGCG4650.020.032457
GATCTCG13200.019.6024596
TTCGGAA3350.019.5678652
Produced by FastQC (version 0.11.2)