FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361037
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16940.12446391979057145No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGGC700.001334009924.6427418
TACGGCC750.001993265222.99989119
GGACCGT953.066122E-421.7893686
CGATCTG33900.020.5574235
CGATCTT19400.019.9174295
ACGGCCG900.00573510819.16657420
GACCGTT1108.2380517E-418.818097
CCGATCT91900.017.7942564
TTCCGAT95400.017.3590472
CTGTCGC2005.9906415E-717.2499189
TCCGATC97250.017.0281963
CGGATAG1452.7229774E-416.65509269
CGGATAC1452.7229774E-416.65509264
ATCAACG1250.001940921616.55992112
TCGTCTG1250.001940921616.55992110
AGCTACG1250.001940921616.55992128
CCGTTAT1250.001940921616.5599219
CTTCCGA101400.016.4679811
TCGCCCA1907.0212154E-616.34202612
TCTGTCG2753.7271093E-916.3090138
Produced by FastQC (version 0.11.2)