FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1536525
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACAT43860.28544930931810414No Hit
GATTAATAGGGATAGTCGGGGGCGTCAGTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACT37960.247050975415304No Hit
GAATAATAGAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGG34590.2251183677453995No Hit
ATAGAATCCCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCC34030.221473780120727No Hit
TTATAGAATCCCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCGTCTTCGATC27850.18125315240559053No Hit
GTATTCAGCTGTCAGAGGTGAAATTCTTGGATTTGCTGAAGACTAACTACTGCGAAAGCATTCGCCAAGGATGTT25280.16452709848521826No Hit
CTCTACTTGTGCGCTATCGGTCTCCGGCCAATATTTAGCTTTAGATGAAATTTACCACCCATTTAGAGCTGCATT24750.16107775662615317No Hit
GGTCATTATAGAATCCCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACGACGGTATCTGATCGTCTT23340.15190120564260262No Hit
GGTTAAGACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACATCCTTGG22750.1480613722523226No Hit
GTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACATCCTTGGCGAATGCTTTCGCAGT21810.14194367159662225No Hit
CTATTATTCCATGCTAATGTATTCGAGCAAAGGCCTGCTTTGAACACTCTAATTTTTTCACAGTAAAAGTCCTGG21610.1406420331592392No Hit
GAATAGGACGTGTGGTTCTATTTTGTTGGTTTCTAGGACCGCCGTAATGATTAATAGGGATAGTCGGGGGCGTCA20260.13185597370690355No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACACGGGCGCGGACACCCCATCCCATACG20120.13094482680073544No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18910.12306991425456792No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCCCAGTAACG17210.11200598753681196No Hit
GTATTTATTGTCACTACCTCCCCGTATCGGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC15670.10198337156896242No Hit
GACTACGACGGTATCTGATCGTCTTCGATCCCCTAACTTTCGTTCCCTGATTAATGAAAACATCCTTGGCGAATG15420.1003563235222336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG8800.030.57927
GCTATCG9500.029.05324713
TGCGCTA9350.028.78135910
GCGCTAT9550.028.1786111
CTACTTG10000.027.9446853
TACTTGT10000.027.9446854
CGCTATC9550.027.81734512
GTGCGCT9700.027.7428559
TGTGCGC10050.027.4623768
ACTTGTG10150.027.1918095
TATCGGT10600.026.03828815
CTTGTGC10850.025.1195326
TCCGGCC11050.024.66648523
CGATCTG40050.023.9472965
CTGTGCG1602.2027962E-923.7192529
CTATCGG12050.023.47767614
GGTTCGC2800.023.41121110
CGGTCTC11600.023.19876918
CGGCCAA11600.022.90134825
CTCCGGC12050.022.3331522
Produced by FastQC (version 0.11.2)