FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67815
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG49197.253557472535575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGT150.002339213669.0501861
CTGATAG150.002339213669.0501861
ACAACTA150.002346074268.9992718
TATATTG150.002346074268.999275
TACATAG150.002346074268.999275
TCCACTG150.002346074268.9992714
GTATATT150.002346074268.999274
AATTACC150.002346074268.9992753
GGGCAAA208.4570864E-568.9992712
CCTTGTA150.002346074268.9992730
CTATAAG200.007308351.787641
CCTTACA200.00732967251.749452
GAGCTGA200.00732967251.749456
TTATGTA200.00732967251.749452
AGAGTTG200.00732967251.749456
AATTGTA200.00732967251.7494555
AGTCTCA200.00732967251.7494521
GCGATAC200.00732967251.7494517
TATAGTC200.00732967251.749452
GGTGACA200.00732967251.7494515
Produced by FastQC (version 0.11.2)