FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56258
Sequences flagged as poor quality0
Sequence length75
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG564410.03235095453091No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCATA150.0023438969.027
GTAGGAC150.0023438969.03
CCCAAAC150.0023438969.052
CCATAGG150.0023438969.029
CAAATTG150.0023438969.037
GTGTAGG150.0023438969.01
AGACAAG150.0023438969.024
GCCTAGA150.0023438969.035
TTTGAAC150.0023438969.031
CACTTGC150.0023438969.057
CGCTTGT150.0023438969.08
TTGTGAG200.007322898651.7532
GCGGTTT200.007322898651.7518
ACGTTCC200.007322898651.757
ACTTGCA200.007322898651.7558
GCGAACT200.007322898651.7538
GAGTCAT200.007322898651.751
CCAATGA200.007322898651.7545
ACAGGGC200.007322898651.758
CTTGCAA200.007322898651.7559
Produced by FastQC (version 0.11.2)