FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228141
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26810.12032452165280384No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG64300.022.2126455
GATCTGG24450.022.0118566
CGATCTT29800.019.4492975
ATCTGCG6350.018.4720927
CCGATCT166700.018.1085364
TTCCGAT174800.017.3878292
TCCGATC177000.017.19123
GTATATA11300.017.0977921
CTTCCGA184050.016.7771261
TGCGCGG2552.60261E-816.23498710
GTATTAG6750.015.8448551
GACCGTC2001.1428792E-515.52505527
TCTGCGC4900.015.4895058
CTGCGCG2907.539711E-915.4652269
CGCCGGG2554.5980596E-714.88207212
TAGACGT8000.014.6622234
CTAGACG5650.014.6545923
ATCTACG3101.8173523E-814.4674697
TATACTT7200.014.3747295
GCTTAGG24300.014.33955769
Produced by FastQC (version 0.11.2)