FastQCFastQC Report
Tue 9 Jan 2018
HW3HFAFXX_n02_zt14ss45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW3HFAFXX_n02_zt14ss45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1578059
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22150.14036230584534545No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG41100.020.6498055
GATCTGG16150.020.080666
CGGATTC1258.7431006E-519.3201669
ATCTCGC4100.018.5123487
CCGATCT104450.018.430984
TTCCGAT108750.017.6076012
TCCGATC110700.017.452723
CGATCTT19900.017.3368265
GTATTAT5600.017.2517811
TCGACTG1402.0825624E-417.25068918
CTTCCGA114200.016.949651
GTATATA8700.016.6568931
CGCAATC1756.358561E-515.77156135
TAGACTG6550.015.2749365
TTATAGT5050.015.0298284
ATCTGCG5050.015.0298287
ACGTTTC1450.0052157414.2759866
GGCGACG1951.5987841E-414.15396365
GTCGCCC1951.5992144E-414.15351511
TTATACT4950.013.9395084
Produced by FastQC (version 0.11.2)